About the Biorepository:
Please note: this section is currently under construction.
The HTRN biospecimen bank is comprised of samples for the Ohio
State University Cancer and Leukemia Group B Pathology Coordinating
Office (CALGB-PCO) and the Ohio State University Midwestern Division
of the Cooperative Human Tissue Network (CHTN) both funded by the National
Cancer Institute.
The CALGB-PCO banks biospecimens donated by patients enrolled in clinical
trials. Samples can include tumor and normal tissue, plasma, serum, whole
blood and white blood cells and urine. All of these samples are used later
in correlative studies.
The Midwestern Division of the CHTN stores a temporary biospecimen bank of
tumor and normal tissue, tissue slides and paraffin embedded tissue blocks
for research investigators throughout the country and Canada who are trying
to find a cure for cancer.
Tissue samples are stored in the vapor phase of liquid nitrogen for long
term storage. Plasma and serums are stored in -80 degree Celsius freezers
and DNA and urines are stored in -20 degree Celsius freezers. Unstained slides
are stored in 4 degree Celsius refrigerators and are vacuum packed to avoid
oxygen degradation.
The biorepository is currently comprised of six liquid nitrogen freezers,
23 -80C freezers, 8 -20C freezers and 17 four degree refrigerators.
As part of the HTRN biospecimen bank, a Rees Scientific equipment monitoring
system helps to secure the integrity and quality of samples stored in the
biorepository. Freezer and refrigerator temperature units are automatically
monitored 24/7 by wireless probes installed in each unit. This system is designed
to provide the ultimate in high-security environmental monitoring. The biorespository
also features an automatic backup electric powered generator and a staff emergency
response team that responds to any unit malfunctions. Unit maintenance checks are
conducted routinely to ensure optimal performance.
Scientific research within the HTRN is currently underway to determine the best
methods in tissue storage for long term use. The NCI First-Generation Guidelines
for NCI-Supported Biorepositories and the NCI Best Practices for Biospecimen Resources
are continuously reviewed and adapted by the HTRN.
